Coding
GFPsf

Part:BBa_K365009:Design

Designed by: RENAULT Renaud   Group: iGEM10_ESBS-Strasbourg   (2010-10-10)


GFP superfolder RFC 25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Design Notes

We deleted the double stop codon to allow fusion assembly.


Source

We extracted the GFP superfolder sequence from the I746915 biobrick, using appropriate primers to add fusion prefix and suffix.

References